Coding

Part:BBa_K5308008:Design

Designed by: Chengyao Yin   Group: iGEM24_SZ-SHD   (2024-10-02)


CscB-SPS-glgC for enhanced sucrose production and secretion from cynaobacteria


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2957
    Illegal EcoRI site found at 4404
    Illegal XbaI site found at 1276
    Illegal PstI site found at 2995
    Illegal PstI site found at 4154
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2957
    Illegal EcoRI site found at 4404
    Illegal NheI site found at 3150
    Illegal NheI site found at 3687
    Illegal PstI site found at 2995
    Illegal PstI site found at 4154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2957
    Illegal EcoRI site found at 4404
    Illegal BglII site found at 4647
    Illegal XhoI site found at 2734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2957
    Illegal EcoRI site found at 4404
    Illegal XbaI site found at 1276
    Illegal PstI site found at 2995
    Illegal PstI site found at 4154
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2957
    Illegal EcoRI site found at 4404
    Illegal XbaI site found at 1276
    Illegal PstI site found at 2995
    Illegal PstI site found at 4154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 525


Design Notes

no


Source

from non-pathogen Escherichia coli and safe Synechococcus elongatus CC 7942

References